Usefulness of conventional blood groups, DNA-minisatellites, and short tandem repeat polymorphisms in paternity testing : a comparison.
A total of 215 paternity cases were analysed after testing 24 marker systems.
Despite technical advantages of polymerase chain reaction related polymorphisms (automatisation, employment of robots, lesser requirements concerning of quality and quantity of DNA) it could be shown that the exclusive employment of a parentage testing kit is compromised by an increased risk of erroneous conclusions.
It is estimated that in about 3-4% of the cases ambiguous situations have to be expected which are caused by the occurrence of single or double exclusions.
In these cases it is impossible to decide whether the exclusions indicate either true nonpaternity or a de novo mutation.
The situation might become even more complicated if an involvement of a close relative of the alleged father cannot be ruled out.
We cautiously advance the hypothesis that in parentage testing DNA minisatellite polymorphisms form an optimal set of tools.
Mots-clés Pascal : Empreinte génomique, Paternité, Traitement, Biologie moléculaire, Groupe sanguin, DNA microsatellite, Marqueur génétique, Aspect médicolégal, Génétique, Séquence répétée en tandem, Polymorphisme, Homme, Etude comparative
Mots-clés Pascal anglais : Genomic imprinting, Paternity, Treatment, Molecular biology, Blood group, Microsatellite DNA, Genetic marker, Forensic aspect, Genetics, Tandemly repeated sequence, Polymorphism, Human, Comparative study
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0410184
Code Inist : 002B30A10. Création : 22/03/2000.