In situations where badly burnt fragments of bone are found, identification of their human or non-human origin may be impossible by gross morphology alone and other techniques have to be employed.
In order to determine whether histological methods were redundant and should be superseded by biomolecular analyses, small fragments of artificially burnt bone (human and non-human) were examined by quantitative and standard light microscopy, and the findings compared with newer biomolecular analyses based on identifying specific human albumin by ELISA and amplifying human mitochondrial DNA by PCR.
For quantitative microscopy, reference data were first created using burnt bones from 15 human and 20 common domestic and farm animals.
Measured osteon and Haversian canal parameters were analysed using multivariate statistical methods.
Highly significant differences were found between values for human and non-human bone, and a canonical discriminant function equation was derived, giving a predicted correct classification of 79%. For the main study, samples of cortical bone were taken from three fresh cadavers, six human skeletons and ten freshly slaughtered animals and burnt by exposure to temperatures ranging from 800 to 1200°C ; charred fragments of human cortical bone from two forensic cases were also tested.
Quantitative microscopy and canonical discriminant function gave the correct origin of every sample. (...)
Mots-clés Pascal : Os, Brûlure, Origine humaine, Identification, Anthropologie, Squelette, Méthode immunologique, Aspect médicolégal, Histologie, DNA, Technique, Etude comparative, Biologie moléculaire, Homme, Evaluation performance, Criminalistique, Police scientifique
Mots-clés Pascal anglais : Bone, Burn, Human origin, Identification, Anthropology, Skeleton, Immunological method, Forensic aspect, Histology, DNA, Technique, Comparative study, Molecular biology, Human, Performance evaluation, Forensic science
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0409059
Code Inist : 002B30A10. Création : 22/03/2000.