In vitro adulterants are used to invalidate assays for urine drugs of abuse.
The present study examined the effect of pyridinium chlorochromate (PCC) found in the product « Urine Luck ».
PCC was prepared and added to positive urine controls at concentrations of 0,10,50, and 100 g/L The controls were assayed for methamphetamine, benzoylecgonine (BE), codeine and morphine, tetrahydrocannabinol (THC), and phencyclidine (PCP) with the Emit II (Syva) and Abuscreen Online (Roche) immunoassays, and by gas chromatography/mass spectrometry (GC/MS).
Two tests were also developed to detect PCC in urine : a spot test to detect chromate ions using 10 g/L 1,5-diphenylcarbazide as the indicator, and a GC/MS assay for pyridine.
We tested 150 samples submitted for routine urinalysis, compliance, and workplace drug testing for PCC, using these assays.
Response rates decreased at 100 g/L PCC for all Emit II drug assays and for the Abuscreen morphine and THC assays.
In contrast, the Abuscreen amphetamine assay produced apparently higher results, and no effect was seen on the results for BE or PCP.
The PCC did not affect the GC/MS recovery of methamphetamine, BE, PCP, or their deuterated internal standards, but decreased GC/MS recovery of the opiates at both intermediate (50 g/L) and high (100 g/L) PCC concentrations and apparent concentrations of THC and THC-d3 at all PCC concentrations. (...)
Mots-clés Pascal : Toxicomanie, Diagnostic, Employé, Lieu travail, Urine, Analyse biochimique, Adultération, Chromate, Additif, Forme commerciale, Interférence, Analyse quantitative, Substance toxicomanogène, Méthode immunologique, Spectrophotométrie, Homme, In vitro, Etats Unis, Amérique du Nord, Amérique, Biologie clinique, Médecine travail, Pyridinium chlorochromate
Mots-clés Pascal anglais : Drug addiction, Diagnosis, Employee, Work place, Urine, Biochemical analysis, Adulteration, Chromates, Additive, Commercial form, Interference, Quantitative analysis, Drug of abuse, Immunological method, Spectrophotometry, Human, In vitro, United States, North America, America, Clinical biology, Occupational medicine, Pyridinium chlorochromate
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0396798
Code Inist : 002B03D. Création : 22/03/2000.