Isolation of Acinetobacter spp including A. baumannii from vegetables : implications for hospital-acquired infections.
A. baumannii is rarely recovered from the skin of patients or healthy European subjects as other genospecies predominate, but it is a significant nosocomial pathogen.
The natural reservoir of this organism is therefore uncertain.
We determined the isolation rates of Acinetobacter spp. from vegetables (as an indicator of the natural environment) using a selective technique and classified the genospecies by amplified ribosomal DNA restriction analysis (ARDRA).
Of the 177 samples of vegetables examined, 30 yielded Acinetobacter, with genospecies 2 and 11 being the most common, each with a frequency of 27%. MIC assays showed that strains of genospecies 1,2,3, and 13TU (the A. calcoaceticus-A. baumannii complex) were significantly more resistant than other genospecies to ciprofloxacin and gentamicin.
Vegetables may therefore be a natural habitat of A. baumannii and provide a route by which these bacteria are introduced into hospitals with obvious implications for infection control.
Mots-clés Pascal : Acinetobacter, Neisseriaceae, Micrococcales, Bactérie, Acinetobacter baumannii, Légume, Exploration microbiologique, Isolement, Fréquence, Fragment restriction, Marqueur RAPD, DNA ribosomique, Environnement, Test sensibilité médicamenteuse, Antibactérien, Aliment, Hygiène
Mots-clés Pascal anglais : Acinetobacter, Neisseriaceae, Micrococcales, Bacteria, Acinetobacter baumannii, Vegetable, Microbiological investigation, Isolation, Frequency, Restriction fragment, Random amplified polymorphic DNA marker, Ribosomal DNA, Environment, Drug susceptibility test, Antibacterial agent, Food, Hygiene
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0373821
Code Inist : 002A05B11. Création : 22/03/2000.