Genetic damage in operating room personnel exposed to isoflurane and nitrous oxide.
Objectives-To evaluate genetic damage as the frequency of sister chromatid exchanges and micronuclei in lymphocytes of peripheral blood of operating room personnel exposed to waste anaesthetic gases.
Methods-Occupational exposure was measured with a direct reading instrument.
Venous blood samples were drawn from 10 non-smokers working in the operating room and 10 non-smoking controls (matched by age, sex, and smoking habits).
Lymphocytes were cultured separately over 72 hours for each assay with standard protocols.
At the end of the culture time, the cells were harvested, stained, and coded for blind scoring.
The exchanges of DNA material were evaluated by counting the number of sister chromatid exchanges in 30 metaphases per probe or by counting the frequency of micronuclei in 2000 binucleated cells.
Also, the mitotic and proliferative indices were measured.
The operating room personnel at the hospital were exposed to an 8 hour time weighted average of 12.8 ppm nitrous oxide and 5.3 ppm isoflurane.
The mean (SD) frequency of sister chromatid exchanges was significantly higher (10.2 (1.9) v 7.4 (2.4)) in exposed workers than controls (p=0.036) the proportion of micronuclei (micronuclei/500 binucleated cells) was also higher (8.7 (2.9) v 6.8 (2.5)), but was not significant (p=0.10).
Conclusion-Exposure even to trace concentrations of waste anaesthetic gases may cause dose-dependent genetic damage. (...)
Mots-clés Pascal : Isoflurane, Azote protoxyde, Anesthésique général, Toxicité, Exposition professionnelle, Médecine travail, Homme, Bloc opératoire, Personnel sanitaire, DNA, Test mutagénicité, Test micronucléus, Echange chromatide soeur, Composé volatil, Halogène Composé organique, Gaz, Lymphocyte
Mots-clés Pascal anglais : Isoflurane, Nitrogen protoxide, General anesthetic, Toxicity, Occupational exposure, Occupational medicine, Human, Operating room, Health staff, DNA, Mutagenicity testing, Micronucleus test, Sister chromatid exchange, Volatile compound, Halogen Organic compounds, Gases, Lymphocyte
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0371057
Code Inist : 002B02U10. Création : 14/12/1999.