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  1. Density and molecular epidemiology of Aspergillus in air and relationship to outbreaks of Aspergillus infection.

    Article - En anglais

    After five patients were diagnosed with nosocomial invasive aspergillosis caused by Aspergillus fumigatus and A. flavus, a 14-month surveillance program for pathogenic and nonpathogenic fungal conidia in the air within and outside the University Hospital in Rotterdam (The Netherlands) was begun.

    A. fumigatus isolates obtained from the Department of Hematology were studied for genetic relatedness by randomly amplified polymorphic DNA (RAPD) analysis.

    This was repeated with A. fumigatus isolates contaminating culture media in the microbiology laboratory.

    The density of the conidia of nonpathogenic fungi in the outside air showed a seasonal variation : higher densities were measured during the summer, while lower densities were determined during the fall and winter.

    Hardly any variation was found in the numbers of Aspergillus conidia.

    We found decreasing numbers of conidia when comparing air from outside the hospital to that inside the hospital and when comparing open areas within the hospital to the closed department of hematology.

    The increase in the number of patients with invasive aspergillosis could not be explained by an increase in the number of Aspergillus conidia in the outside air.

    The short-term presence of A. flavus can only be explained by the presence of a point source, which was probably patient related.

    Genotyping A. fumigatus isolates from the department of hematology showed that clonally related isolates were persistently present for more than 1 year. (...)

    Mots-clés Pascal : Aspergillus fumigatus, Fungi Imperfecti, Fungi, Thallophyta, Milieu hospitalier, Pollution air, Contamination biologique, Epidémiologie moléculaire, Typage, Génotype, Marqueur RAPD, Conidie, Analyse quantitative, Variation saisonnière, Surveillance sanitaire, Pays Bas, Europe

    Mots-clés Pascal anglais : Aspergillus fumigatus, Fungi Imperfecti, Fungi, Thallophyta, Hospital environment, Air pollution, Biological contamination, Molecular epidemiology, Typing, Genotype, Random amplified polymorphic DNA marker, Conidia, Quantitative analysis, Seasonal variation, Sanitary surveillance, Netherlands, Europe

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 99-0354971

    Code Inist : 002A05D05. Création : 14/12/1999.