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  1. Semen specific gamma-glutamyltransferase carries ABH antigens : a sandwich ELISA for simultaneous semen detection and its ABO blood typing.

    Article - En anglais

    Semen type of gamma-glutamyltransferase (gamma-GTP) is different from the membrane bound type of the enzyme in both biochemical and immunological properties, and consists of two subunits (150 and 95 kDa).

    We found that anti-ABH antibodies recognize a 150-kDa subunit of seminal gamma-GTP by Western blot and immunoprecipitation analyses.

    Using SG2, one of anti-semen specific gamma-GTP monoclonal antibodies which we had produced, and anti-ABH antibodies, we established a sandwich ELISA for identifying human seminal gamma-GTP and its ABO type simultaneously.

    This sandwich ELISA allows ABO typing of highly diluted semen.

    The dilutions for ABO typing were 105 times for A or O, and 104 times for B. Furthermore, ABO typing of semen was successfully performed by this ELISA, even in the mixed presence of vaginal fluid, saliva and blood.

    Thus, seminal gamma-GTP carries ABH antigens and the sandwich ELISA with SG2 and anti-ABH antibodies enables ABO typing of semen.

    The sandwich ELISA is extremely useful for ABO typing originated from semen in the mixture of biological fluids.

    Mots-clés Pascal : Sperme, Liquide biologique, Groupage sanguin, Groupe sanguin, Système ABO(H), gamma-Glutamyltransferase, Aminoacyltransferases, Transferases, Enzyme, Technique ELISA, Liquide séminal, Homme, Mâle, Médecine légale, Victimologie, Biologie clinique, Technique sandwich

    Mots-clés Pascal anglais : Semen, Biological fluid, Blood typing, Blood group, ABO(H)-System, gamma-Glutamyltransferase, Aminoacyltransferases, Transferases, Enzyme, ELISA assay, Seminal plasma, Human, Male, Legal medicine, Victimology, Clinical biology, Sandwich technique

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 99-0352904

    Code Inist : 002B24O11. Création : 14/12/1999.