Semen specific gamma-glutamyltransferase carries ABH antigens : a sandwich ELISA for simultaneous semen detection and its ABO blood typing.
Semen type of gamma-glutamyltransferase (gamma-GTP) is different from the membrane bound type of the enzyme in both biochemical and immunological properties, and consists of two subunits (150 and 95 kDa).
We found that anti-ABH antibodies recognize a 150-kDa subunit of seminal gamma-GTP by Western blot and immunoprecipitation analyses.
Using SG2, one of anti-semen specific gamma-GTP monoclonal antibodies which we had produced, and anti-ABH antibodies, we established a sandwich ELISA for identifying human seminal gamma-GTP and its ABO type simultaneously.
This sandwich ELISA allows ABO typing of highly diluted semen.
The dilutions for ABO typing were 105 times for A or O, and 104 times for B. Furthermore, ABO typing of semen was successfully performed by this ELISA, even in the mixed presence of vaginal fluid, saliva and blood.
Thus, seminal gamma-GTP carries ABH antigens and the sandwich ELISA with SG2 and anti-ABH antibodies enables ABO typing of semen.
The sandwich ELISA is extremely useful for ABO typing originated from semen in the mixture of biological fluids.
Mots-clés Pascal : Sperme, Liquide biologique, Groupage sanguin, Groupe sanguin, Système ABO(H), gamma-Glutamyltransferase, Aminoacyltransferases, Transferases, Enzyme, Technique ELISA, Liquide séminal, Homme, Mâle, Médecine légale, Victimologie, Biologie clinique, Technique sandwich
Mots-clés Pascal anglais : Semen, Biological fluid, Blood typing, Blood group, ABO(H)-System, gamma-Glutamyltransferase, Aminoacyltransferases, Transferases, Enzyme, ELISA assay, Seminal plasma, Human, Male, Legal medicine, Victimology, Clinical biology, Sandwich technique
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0352904
Code Inist : 002B24O11. Création : 14/12/1999.