To explore the possible oxidative stress induced by lead, heparinized whole blood and urine of 66 secondary smelter lead workers (46 for Comet assay) and 28 controls were collected.
The concentrations of blood lead (BPb) and urinary lead (UPb) and alpha-amino-levulinic acid (alpha-ALA), indices of lead exposure level of the body, were determined.
Malondialdehyde (MDA) concentrations and superoxide dismutase (SOD) activity of plasma were also measured.
Single-cell gel (SCG, Comet assay) was used to measure the DNA damage of peripheral blood cells.
There was a positive correlation between the presence of Pb in blood and significant increases in MDA levels and SOD activity.
Alcohol consumption and smoking with increased exposure to Pb was associated with enhanced DNA damage.
A positive correlation was found between MDA and DNA damage.
Mots-clés Pascal : Toxicité, DNA, Carcinogène, Métal lourd, Plomb, Médecine travail, Exposition professionnelle, Industrie métallurgique, Homme, Test comète, Stress oxydatif, Mécanisme action, Superoxide dismutase, Oxidoreductases, Enzyme, Liquide biologique, Urine, Sang, Erythrocyte, Propanedial
Mots-clés Pascal anglais : Toxicity, DNA, Carcinogen, Heavy metal, Lead, Occupational medicine, Occupational exposure, Metallurgical industry, Human, Comet assay, Oxidative stress, Mechanism of action, Superoxide dismutase, Oxidoreductases, Enzyme, Biological fluid, Urine, Blood, Red blood cell
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0348414
Code Inist : 002B30B01A. Création : 14/12/1999.