Micronucleus assay combined with Giemsa and DAPI staining was performed on blood samples of subjects occupationally exposed to ultrasound.
Lymphocytes were cultivated in vitro for 72 h. At 44h cytochalasin-B was added in cultures.
Frequencies of micronuclei in exposed subjects statistically significant increased compared to control.
The frequency of micronucleated cells and micronuclei in exposed subjects shows interindividual variability.
Using DAPI staining we observed signal-positive and signal-negative micronuclei.
Percentage of signal-positive micronuclei varies between 0 and 66.7% and signal-negative micronuclei between 33.3% and 100%. This study indicate harmful effects of ultrasound on human genome, but further investigations are necessary.
Mots-clés Pascal : Ultrason, Toxicité, Effet biologique, Exposition professionnelle, Médecine travail, Homme, Test mutagénicité, Test micronucléus
Mots-clés Pascal anglais : Ultrasound, Toxicity, Biological effect, Occupational exposure, Occupational medicine, Human, Mutagenicity testing, Micronucleus test
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Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0338810
Code Inist : 002A08E. Création : 14/12/1999.