This supercritical fluid extraction study determined the retentive properties of neutral alumina sorbent as an in-line trap for lipids in the dynamic state over a pressure range of 490-680 bar and temperatures of 40 and 80°C. Lipids were extracted from a chicken liver matrix using supercritical carbon dioxide over a 40-min period at a flow rate of 3 L/min (expanded gas), then were quantified by high-performance liquid chromatography using an evaporative light-scattering detector.
Approximately 30 and 18%, respectively, of the total extracted lipids were trapped on the in-line alumina sorbent bed at 40°C as the operating pressure increased from 490 to 680 bar, while the remaining lipids were trapped off-line after CO2 decompression.
The major lipid classes trapped in-line were fatty acids and cholesterol, whereas only minor amounts of the less polar lipid classes such as sterol esters and triacylglycerols were retained.
At 80°C and 680 bar, less than 1.5% of the extracted total lipids was trapped in-line, indicating the lack of adsorptive selectivity for lipids by alumina under these conditions.
Mots-clés Pascal : Extraction SFE, Acide gras, Cholestérol, Piégeage, Lipide, Poulet, Aves, Vertebrata, Foie, Solvant supercritique, Carbone dioxyde, Alumine, Chromatographie SFC, Procédé extraction, Méthode analytique, Sorbant
Mots-clés Pascal anglais : Supercritical fluid extraction, Fatty acids, Cholesterol, Trapping, Lipids, Chicken, Aves, Vertebrata, Liver, Supercritical solvent, Carbon dioxide, Alumina, Supercritical fluid chromatography, Extraction process, Analytical method, Sorbent
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0335467
Code Inist : 002A35B05. Création : 16/11/1999.