When administered to mice, either orally or intraperitoneally, extracts of the cyanobacterium Cylindrospermopsis raciborskii strain AWT 205 induced dose-dependent liver and kidney damage.
Liver damage was generally centrilobular, becoming more severe and generalized as the dose increased.
Damaged hepatocytes were characterized by increased cellular vacuolation, intercellular spaces, and darker nuclear and cytoplasmic staining.
Kidney damage was characterized by a reduction in the number of erythrocytes in the glomerulus and an increase in the space around the glomerulus, increased diameter of the tubule lumina, proximal tubule epithelial necrosis, and the presence of proteinaceous material in the distal tubules.
Transmission electron microscopy of the same tissue revealed epithelial cell necrosis in the proximal tubules, suggesting the material accumulating in the distal tubules was in part cell debris from this necrosis.
The nature, location, and time course of histological damage were similar for oral and intraperitoneal administration, with maximum damage being observed 2-3 days after treatment.
The LD50 (24 h) for intraperitoneally administered Cylindrospermopsis preparations ranged from 50 to 110 mg dry weight of lysed cells per kilogram, whereas the LD50 (7 days) ranged between 20 and 65 mg/kg depending upon the batch examined. (...)
Mots-clés Pascal : Toxicité, Foie, Rein, Intoxication, Voie orale, Voie intrapéritonéale, Souris, Rodentia, Mammalia, Vertebrata, Alcaloïde, Australie, Océanie, Etude comparative, Santé et environnement, Mâle, Animal laboratoire, Cyanobacteria, Bactérie, Toxine, Cylindrospermopsis raciborskii, Cylindrospermopsine
Mots-clés Pascal anglais : Toxicity, Liver, Kidney, Poisoning, Oral administration, Intraperitoneal administration, Mouse, Rodentia, Mammalia, Vertebrata, Alkaloid, Australia, Oceania, Comparative study, Health and environment, Male, Laboratory animal, Cyanobacteria, Bacteria, Toxin
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0327085
Code Inist : 002A05B10. Création : 16/11/1999.