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  1. Detection of polycyclic aromatic hydrocarbon metabolites by high-pressure liquid chromatography after purification on immunoaffinity columns in urine from occupationally exposed workers.

    Article - En anglais

    Objective 

    The objective in our study was to quantitate benzo[a]pyrene (B[a]P) metabolites by a combination of immunoaffinity chromatography and high-pressure liquid chromatography (HPLC) with fluorescence detection in urine from workers exposed to high levels of polycyclic aromatic hydrocarbons (PAH).

    Furthermore, by the simultaneous quantitation of 1-hydroxypyrene, the correlation between the B[a]P-tetrol and 1-hydroxypyrene would provide a means of evaluating the validity of 1-hydroxypyrene as a surrogate biomarker for occupational exposure to the potent carcinogen B[a]P in an electrode paste plant.

    Methods 

    The study was carried out at an electrode paste plant that produces electrode paste for Söderberg electrodes.

    A total of 34 pre-and post-shift urine samples and 17 personal air samples were collected from 17 workers during a normal work week.

    The concentration of 1-hydroxypyrene was measured in all urine samples.

    A recent method of quantitating B[a]P-r-7, t-8, t-9, c-10-tetrol in urine of humans exposed to low levels of PAH has been described.

    A modified version of this method involving purification of urine samples on immunoaffinity columns and HPLC analysis with fluorescence detection was used on urine samples from workers exposed to high levels of PAH.

    A monoclonal antibody (8E11) with binding affinity to B[a]P-tetrols was used.

    This antibody also binds several PAH-DNA adducts and metabolites, including 1-hydroxypyrene. (...)

    Mots-clés Pascal : Exposition professionnelle, Médecine travail, Composé aromatique polycyclique, Métabolite, Surveillance biologique, Méthodologie, Analyse quantitative, Liquide biologique, Urine, Marqueur biologique, Chromatographie immunoaffinité, Chromatographie HPLC, Homme, Industrie chimique, Teneur air ambiant, Lieu travail, Pyrène(1-hydroxy)

    Mots-clés Pascal anglais : Occupational exposure, Occupational medicine, Polycyclic aromatic compound, Metabolite, Biological monitoring, Methodology, Quantitative analysis, Biological fluid, Urine, Biological marker, Immunoaffinity chromatography, HPLC chromatography, Human, Chemical industry, Ambient air concentration, Work place

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 99-0289430

    Code Inist : 002B03L06. Création : 16/11/1999.