Low level saliva cotinine determination and its application as a biomarker for environmental tobacco smoke exposure.
Passive Smoking & Children : Clinical & Experimental Forums. International Academic Symposium. Essen, DEU, 1998/08/25.
1 The determination of personal exposures to environmental tobacco smoke (ETS) and respirable suspended particles (RSP) for housewives, and fixed site monitoring of their homes, have been undertaken by these authors throughout Europe, South East Asia and Australia.
Median 24 h time weighted average (TWA) concentrations for ETS particles and nicotine were found to be significantly higher for housewives living in smoking households compared with those living in nonsmoking households.
For Europe, median TWA concentrations of 4.1 and<0.26 mug/m3 for ETS particles and 0.63 and<0.08 mug/m3 for nicotine were found for housewives living in smoking and nonsmoking households respectively. 2 In addition to the measurement of RSP, ETS particles and nicotine, saliva cotinine concentrations were determined using a radioimmunoassay method with a limit of quantitation of 1 ng/ml.
Median saliva cotinine concentrations of 1.4 and<1 ng/ml were determined for European housewives living in smoking and nonsmoking households respectively, which reflected the poor limit of quantitation of this methodology.
A chromatographic method utilising tandem mass-spectrometric detection was developed and validated for the determination of both cotinine and 3-hydroxycotinine, two of the main metabolites of nicotine, with lower limits of quantitation of 0.05 and 0.10 ng/ml respectively. (...)
Mots-clés Pascal : Tabagisme passif, Tabagisme, Toxicité, Particule en suspension, Pollution air, Etude multicentrique, Marqueur biologique, Détection, Liquide biologique, Salive, Taux, Faible, Cotinine, Homme, Femelle, Epidémiologie, Enfant
Mots-clés Pascal anglais : Passive smoking, Tobacco smoking, Toxicity, Suspended particle, Air pollution, Multicenter study, Biological marker, Detection, Biological fluid, Saliva, Rate, Low, Cotinine, Human, Female, Epidemiology, Child
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0260053
Code Inist : 002B03E. Création : 16/11/1999.