Within the frame of a continuing interest in occupational hygiene of hospitals as workplaces, we describe an automated analytical method by reversed-phase high-performance liquid chromatography for the measurement of contamination from the three most important nucleoside analogue antineoplastic drugs (5-fluorouracil, 5FU ; cytarabin, CYA ; gemcytabin, GCA) on such surfaces as those of preparation hoods and work-benches in departmental pharmacies of oncologic departments.
Our method is characterized by a short analysis time (7 min) under isocratic conditions, by the use of a mobile phase devoid of organic solvent and by high sensitivity (LOD>=40 mug/l for all compounds), adequate to detect surface contamination above a threshold of 4 mug/m2 for wide surfaces and of 30 mug/m2 for small irregular objects.
We present some results from a preliminary survey study recently performed in seven oncologic departments of two large general hospitals in Milan.
To exemplify the contamination levels on various surfaces (such as on handles, floor surfaces and window glass panes, even far from the preparation hood), analyte concentrations in the order of 0.03-0.06 mug/ml, corresponding to 0.8-1.5 mug of 5FU were measured on telephones, of 0.02-0.6 mug/ml (0.85-28 mug/m2) of CYA were measured on table boards, of 0.05-10.6 mug/ml (1.2-1150 mug/m2) of GCA on furniture and floors.
Spillage fractions up to 1% of the employed ANDs.
(employed daily 5FU 7-13 g ; CYA 0.1-7.1 g ; (...)
Mots-clés Pascal : Cytarabine, Pyrimidine nucléoside, Fluorouracil, Fluoropyrimidine dérivé, Anticancéreux, Contamination, Surface contact, Service hospitalier, Hygiène, Analyse quantitative, Chromatographie HPLC, Chromatographie phase inverse, Détecteur UV, Italie, Europe, Gemcytabine
Mots-clés Pascal anglais : Cytarabine, Pyrimidine nucleoside, Fluorouracil, Fluoropyrimidine derivatives, Antineoplastic agent, Contamination, Contact surface, Hospital ward, Hygiene, Quantitative analysis, HPLC chromatography, Reversed phase chromatography, Ultraviolet detector, Italy, Europe
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0244203
Code Inist : 002B02A02. Création : 16/11/1999.