While in the United States and northern Europe, human herpesvirus 8 (HHV-8) appears to be mainly sexually transmitted with primary infection occurring in adulthood, the modes of transmission remain unknown in East and Central Africa, where Kaposi's sarcoma (KS) is a long-standing endemic disease, occurring not only in adults but also in children.
The aim of our present study was to determine the prevalence of HHV-8 infection in children from Yaoundé, Cameroon, Central Africa.
Specific antibodies directed against both latent and lytic HHV-8 antigens were detected and titrated, with an immunofluorescence assay using the KS-I cell line, in the plasma of 258 children and adolescents, of 32 mother and child pairs and of 189 pregnant women.
Two different HHV-8 DNA-specific sequences were searched in the buffy coat by PCR assays.
The overall HHV-8 seroprevalence was 27.5% among these children and adolescents.
In newborns, seroprevalence reached 46%, reflecting passive transmission of maternal IgG.
This was followed by a marked drop.
Then, beginning around 4 years of age, a regular increase of HHV-8 antibodies took place, reaching 39% in the 12-to 14-year age group and 48% above 15 years, a rate similar (54.5%) to that observed in pregnant women.
PCR detection of HHV-8 sequences was negative in seronegative children and positive in the buffy coat in 17% of HHV-8-seropositive children, reflecting a low viral load in the peripheral blood. (...)
Mots-clés Pascal : Herpès, Virose, Infection, Prévalence, Epidémiologie, Cameroun, Afrique, Sérologie, Exploration immunologique, Réaction chaîne polymérase, Enfant, Homme, Gammaherpesvirinae, Herpesviridae, Virus, Biologie moléculaire, Rhadinovirus, Herpesvirus hominis 8
Mots-clés Pascal anglais : Herpes, Viral disease, Infection, Prevalence, Epidemiology, Cameroon, Africa, Serology, Immunological investigation, Polymerase chain reaction, Child, Human, Gammaherpesvirinae, Herpesviridae, Virus, Molecular biology, Herpesvirus hominis 8
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0244111
Code Inist : 002B05C02J. Création : 16/11/1999.