Enhanced basophil histamine release and neutrophil chemotactic activity predispose grain dust-induced airway obstruction.
Background The pathogenic mechanism of grain dust (GD) - induced occupational asthma (OA) remains unclear.
Objective To understand further the mechanism of GD-induced OA.
Methods Fifteen employees working in a same GD industry, complaining of work-related respiratory symptoms, were enrolled and were divided into two groups according to the GD-bronchoprovocation test (BPT) result : six positive responders were grouped as group III, nine negative responders as group II and five healthy controls as group I. Serum GD-specific immunoglobulin (Ig) E (sIgE), specific IgG (sIgG) and specific IgG4 (sIgG4) antibodies were detected by enzyme-linked immunosorbent assay.
Basophil histamine release was measured by the autofluorometric method, and changes of serum neutrophil chemotactic activity were observed by the Boyden chamber method.
Results For clinical parameters such as degree of airway hyperresponsiveness to methacholine, duration of respiratory symptoms, exposure duration, and prevalences of serum sIgE, sIgG and sIgG4 antibodies, there were no significant differences between group II and III (P>0.05, respectively).
Serum neutrophil chemotactic activity increased significantly at 30min and decreased at 240min after the GD-BPT in group III subjects (P<0.05, respectively), while no significant changes were noted in group II subjects (P>0.05).
Basophil histamine release induced by GD was significantly higher in group III than those of group I or group II (...)
Mots-clés Pascal : Grain, Poussière, Exposition professionnelle, Asthme, Pathogénie, Granulocyte, Basophile, Histamine, Neutrophile, Chimiotactisme, Homme, Médecine travail, Appareil respiratoire pathologie, Bronchopneumopathie obstructive
Mots-clés Pascal anglais : Grains, Dust, Occupational exposure, Asthma, Pathogenesis, Granulocyte, Basophil, Histamine, Neutrophil, Chemotaxis, Human, Occupational medicine, Respiratory disease, Obstructive pulmonary disease
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0241322
Code Inist : 002B11B. Création : 16/11/1999.