Objective Human papillomavirus infection is an important aetiological agent associated with the development of cervical neoplasia.
However, even with the most sensitive methods of detection, human papillomavirus DNA has been detected in only 90% of cases of cervical cancer and between 80% - 90% of cases of dysplasia.
This study aimed to determine if there are epidemiological differences between women who are positive or negative for human papillomavirus, with high grade cervical intraepithelial neoplasia (CIN).
Design Four hundred and sixty women with CIN II and III lesions were studied.
To ensure optimal detection of human papillomavirus DNA, two specimens (i.e. tampon and cervical biopsy) were collected from each woman and tested by three techniques : L1-polymerase chain reaction, E6-PCR and low stringency Southern blotting.
A detailed questionnaire was completed and blood sample collected for determination of serum levels of beta-carotene, vitamin A and E from each patient.
Human leucocyte antigen (HLA) - DQB 1 alleles were also compared between the groups of women who were positive or negative for human papillomavirus.
Results Overall, human papillomavirus DNA analysis was positive in 411 women (89%). Age, number of sexual partners in the last 12 months, past pregnancy and marital status were associated with human papillomavirus detection in the crude analysis. (...)
Mots-clés Pascal : Tumeur maligne, Col utérus, Haute malignité, Papillomavirus humain, Papillomavirus, Papovaviridae, Virus, Etude comparative, Etiologie, Homme, Femelle, Epidémiologie, Appareil génital femelle pathologie, Col utérus pathologie
Mots-clés Pascal anglais : Malignant tumor, Uterine cervix, High malignancy, Human papillomavirus, Papillomavirus, Papovaviridae, Virus, Comparative study, Etiology, Human, Female, Epidemiology, Female genital diseases, Uterine cervix diseases
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0196676
Code Inist : 002B20C02. Création : 16/11/1999.