In this study, three types of treated wastewater were tested for infectious enteroviruses, the enterovirus genome, somatic coliphages, and Bacteroides fragilis phages.
The aim of this work was to determine whether the presence of the two types of bacteriophages or of the enterovirus genome was a good indicator of infectious enterovirus contamination.
The enterovirus genome was detected by reverse transcription-polymerase chain reaction.
Infectious enteroviruses were quantified by cell culturing (BGM cells), and the bacteriophages were quantified by plaque formation on the host bacterium (Escherichia coli or B. fragilis) in agar medium.
Forty-eight samples of treated wastewater were analyzed.
Sixteen samples had been subjected to a secondary treatment for 8 to 12 h (A), 16 had been subjected to a secondary treatment for 30 h (B1), and 16 had been subjected to both secondary and tertiary treatments (B2).
The mean concentrations of somatic coliphages were 4.9 x 104 PFU. liter-1 for treatment line A, 9.8 x 103 PFU. liter-1 for B1, and 1.4 x 103 PFU. liter-1 for B2, with all the samples testing positive (100%). The mean concentrations of B. fragilis phages were 1.7 x 103 PFU. liter-1 for A (100% positive samples), 17 to 24 PFU. liter-1 for B1 (44% positive samples), and 0.8 to 13 PFU. liter-1 for B2 (6% positive samples).
The mean concentrations of infectious enteroviruses were 4 most probable number of cytopathogenic units (MPNCU). (...)
Mots-clés Pascal : Eau usée, Contamination biologique, Enterovirus, Picornaviridae, Virus, Indicateur biologique, Coliphage, Bactériophage, Epuration eau usée, Bacteroides fragilis, Bacteroidaceae, Bactérie, Génome, Qualité eau, Détection, Méthode, Pollution eau
Mots-clés Pascal anglais : Waste water, Biological contamination, Enterovirus, Picornaviridae, Virus, Biological indicator, Coliphage, Phage, Waste water purification, Bacteroides fragilis, Bacteroidaceae, Bacteria, Genome, Water quality, Detection, Method, Water pollution
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0187934
Code Inist : 002A05C09. Création : 16/11/1999.