Mutational activation of K-ras in nonneoplastic exocrine pancreatic lesions in relation to cigarette smoking status.
Cigarette smoking is among the few unequivocal risk factors for the development of pancreatic ductal adenocarcinoma (PDAC).
Activating mutations in codon 12 of the K-ras protooncogene is a frequent and early molecular event in the pathogenesis of PDAC and a variety of nonmalignant ductal pancreatic lesions.
The molecular epidemiologic relation between heavy cigarette smoking and mutational activation of K-ras in PDAC has been examined to a limited extent.
The authors have examined the mutational status of K-ras in nonneoplastic pancreata in relation to cigarette smoking status.
Archival formalin fixed paraffin embedded specimens of nonneoplastic pancreata (n=39) were obtained from the American Cancer Society and evaluated histopathologically.
Specimens from age-and gender-matched individuals were stratified into three groups : 1) those who never smoked cigarettes (n=16), 2) those who smoked 1-2 packs/day for more than 20 years (n=10 cases), and 3) those who smoked more than 2 packs/day for 20 or more years (n=13).
Cases were preselected from 77 specimens based on the quality, suitability, and cellularity of the archival tissues for analyses.
Furthermore, none of the patients died of primary PDAC or had evidence of pancreatic metastases from an extrapancreatic primary tumor.
Tissue sections were microdissected and deparaffinized, and genomic DNA was purified by standard proteinase K-phenol-chloroform extraction techniques. (...)
Mots-clés Pascal : Protooncogène, Gène onc cellulaire, Mutation, Métaplasie, Fibrose, Pancréas exocrine, Adénocarcinome, Facteur risque, Epidémiologie, Etats Unis, Amérique du Nord, Amérique, Homme, Génétique, Pancréas pathologie, Appareil digestif pathologie, Lésion précancéreuse, Tumeur maligne, Gène K ras, Gène ras
Mots-clés Pascal anglais : Protooncogene, C-Onc gene, Mutation, Metaplasia, Fibrosis, Exocrine pancreas, Adenocarcinoma, Risk factor, Epidemiology, United States, North America, America, Human, Genetics, Pancreatic disease, Digestive diseases, Premalignant lesion, Malignant tumor
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0117633
Code Inist : 002B13C01. Création : 16/11/1999.