To determine whether haematopoietic progenitor cells and erythropoietin (EPO), which is an essential humoral stimulus for erythroid progenitor (BFU-E) cell differentiation, are affected by lead intoxication.
Methods-In male subjects chronically exposed to lead with and without anaemia, pluripotent (CFU-GEMM), BFU-E and granulocyte/macrophage (CFU-GM) progenitor cell counts in peripheral blood were measured with a modified clonal assay.
Lead concentrations in blood (PbB) and urine (PbU) were measured by the atomic absorption technique, and EPO was measured with a modified radioimmunoassay.
PbB in the subjects exposed to lead ranged from 0.796 to 4.4 mumol/l, and PbU varied between 0.033 and 0.522 mumol/l. In subjects exposed to lead with PbB >= 2.896 mumol/l (n=7), BFU-E cells were significantly reduced (p<0.001) whereas the reduction in CFU-GM cells was only of borderline significance (p=0.037) compared with the age matched controls (n=20).
Furthermore, BFU-E and CFU-GM cells were reduced in a dose dependent fashion, with increasing PbB or PbU, respectively.
In the subjects exposed to lead EPO was in the normal range and did not increase in the presence of anaemia induced by lead.
No correlations existed between EPO and PbB, PbU, or progenitor cells. (...)
Mots-clés Pascal : Plomb, Métal lourd, Exposition professionnelle, Médecine travail, Industrie, Cellule BFU-E, Toxicité, Homme, Erythropoïétine, Surveillance biologique, Marqueur biologique, Liquide biologique, Sang, Etude cas témoin
Mots-clés Pascal anglais : Lead, Heavy metal, Occupational exposure, Occupational medicine, Industry, BFU-E-Cell, Toxicity, Human, Erythropoietin, Biological monitoring, Biological marker, Biological fluid, Blood, Case control study
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0108998
Code Inist : 002B03L05. Création : 16/11/1999.