Prevalence of autoantibodies to ribosomal P proteins in juvenile-onset systemic lupus erythematosus compared with the adult disease.
To determine the prevalence of anti-ribosomal P (anti-P) proteins in several groups of patients with juvenile-onset systemic lupus erythematosus (SLE) in comparison with the prevalence in adult SLE.
Serum samples were pooled together from 3 cohorts of patients with juvenile-onset SLE in 3 different medical centers and from a miscellaneous group of juvenile-onset SLE patients whose samples were sent by regional physicians.
Sera were studied for the presence of anti-P using 2 assays : Western blot with ribosomes as antigen, and an enzyme-linked immunosorbent assay with the COOH-terminal 22 amino acids of the ribosomal P protein in a multiantigenic peptide format as antigen.
Sera found positive by both tests were considered positive for anti-P antibodies.
Findings from similar studies involving a large cohort of patients with adult-onset SLE from Oklahoma City were used for comparison.
The prevalence of anti-P antibodies in the pooled sample of juvenile-onset SLE sera was 45 of 108, or 42%, while in the adult cohort from Oklahoma City, 20 of 260, or 7.7%, were positive for anti-P (odds ratio [OR] 9.6, P<10-8 by Fisher's exact test).
In addition, it was shown that 12 of 13 patients with both anti-P and anti-double-stranded DNA (anti-dsDNA) in the juvenile SLE cohort had nephritis, while only 8 of 22 patients without both antibodies were nephritic (OR 21.0, P<10-8). (...)
Mots-clés Pascal : Lupus érythémateux, Disséminé, Age apparition, Enfant, Homme, Adulte, Etude comparative, Epidémiologie, Prévalence, Anticorps, Autoanticorps, Protéine ribosomique, Sérum, Technique ELISA, Symptomatologie, Peau pathologie, Tissu conjonctif pathologie, Maladie système, Maladie autoimmune, Immunopathologie, Protéine ribosomique P
Mots-clés Pascal anglais : Lupus erythematosus, Disseminated, Age of onset, Child, Human, Adult, Comparative study, Epidemiology, Prevalence, Antibody, Autoantibody, Ribosomal protein, Serum, ELISA assay, Symptomatology, Skin disease, Connective tissue disease, Systemic disease, Autoimmune disease, Immunopathology
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0103133
Code Inist : 002B07. Création : 16/11/1999.