Evaluation of enzyme-linked immunosorbent assay for the determination of polycyclic aromatic hydrocarbons in house dust and residential soil.
Two commercially available enzyme-linked immunosorbent assays (ELISA) for total polycyclic aromatic hydrocarbon (PAH) and carcinogenic PAH (C-PAH) were evaluated.
The testing procedures were refined for application to screening PAH and C-PAH in house dust and soil samples for human exposure studies.
The overall method precision expressed as percent relative standard deviation (% RSD) of triplicate real world dust and soil samples was within ± 29% (12-29%) for PAH ELISA and ± 21% (5.9-21%) for C-PAH ELISA.
Spike recoveries from real world dust/soil samples were 114 ± 30% for phenanthrene from PAH ELISA and 120 ± 8.2% for benzo[a]pyrene from C-PAH ELISA.
The overall method accuracy for PAH and C-PAH assays cannot be assessed for multiple PAH components in dust/soil samples (which represent real-world samples), because of the assays'cross reactivities with other PAH components.
Over 100 dust/soil samples from 13 North Carolina homes and 22 Arizona homes were analyzed by PAH and C-PAH assays, as well as by the conventional gas chromatography/mass spectrometry (GC/MS) method.
Statistical analysis showed that dust/soil PAH data from ELISA and GC/MS methods are significantly different.
In general PAH ELISA responses were higher than PAH GC/MS responses.
The regression analysis showed that the linear relationship between ELISA and GC/MS measurements is not strong in the combined data.
The relationship became stronger for the data from the same type of dust/soil samples. (...)
Mots-clés Pascal : Arizona, Etats Unis, Amérique du Nord, Amérique, Caroline du Nord, Pollution intérieur, Logement habitation, Poussière maison, Pollution sol, Analyse chimique, Teneur, Composé aromatique polycyclique, Hydrocarbure, Technique ELISA, Méthode immunoenzymatique, Analyse quantitative, Précision mesure
Mots-clés Pascal anglais : Arizona, United States, North America, America, North Carolina, Indoor pollution, Housing, House dust, Soil pollution, Chemical analysis, Content, Polycyclic aromatic compound, Hydrocarbon, ELISA assay, Enzyme immunoassay, Quantitative analysis, Measurement accuracy
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0090201
Code Inist : 001D16C06. Création : 31/05/1999.