Molecular confirmation of hepatitis A virus from well water : Epidemiology and public health implications.
An outbreak of hepatitis A in a rural river-island community was found to be associated with consumption of contaminated well water.
Specimens from case-patients, the implicated well, and a cesspool suspected to be the source of contamination were all positive for hepatitis A virus (HAV) RNA by immunocapture reverse-transcription polymerase chain reaction.
All isolates were identical over about 400 bases from two capsid-encoding regions of the genome, identifying the chain of transmission.
Other wells up to 60 m from the cesspool also contained HAV RNA.
In addition, HAV RNA was detected in the contamination source well 6 months after the initial contamination, when fecal coliform bacteria were no longer present.
These findings demonstrate the utility of viral detection techniques to evaluate contaminated ground water.
Mots-clés Pascal : Virus hépatite A, Hepatovirus, Picornaviridae, Virus, Homme, Epidémiologie moléculaire, Puits eau, Répartition géographique, Contamination biologique, Contamination, Diagnostic, Hépatite virale A, Virose, Infection, Epidémie, Appareil digestif pathologie, Foie pathologie
Mots-clés Pascal anglais : Hepatitis A virus, Hepatovirus, Picornaviridae, Virus, Human, Molecular epidemiology, Water well, Geographic distribution, Biological contamination, Contamination, Diagnosis, Viral hepatitis A, Viral disease, Infection, Epidemic, Digestive diseases, Hepatic disease
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0075612
Code Inist : 002A05C06. Création : 31/05/1999.