DNA fingerprinting establishes the genetic relatedness of Mycobacterium tuberculosis isolates and has become a powerful tool in tuberculosis epidemiology.
To use DNA fingerprinting to assess the efficacy of current tuberculosis infection-control practices.
Retrospective molecular and descriptive epidemiologic study.
A 700-bed urban public hospital that follows the Centers for Disease Control and Prevention (CDC) guidelines for tuberculosis infection control.
183 patients who had positive cultures for M. tuberculosis from 1 April 1995 to 31 March 1996.
173 of 183 M. tuberculosis isolates from the study period underwent DNA fingerprinting.
Fingerprinting revealed that five isolates represented false-positive cultures and that 91 (54%) of the remaining 168 isolates were in 15 DNA fingerprinting clusters, which ranged in size from 2 to 29 isolates.
Risk factors for clustering were birth in the United States, African-American ethnicity, homelessness, substance abuse, and male sex.
Retrospective epidemiologic analysis of inpatient and outpatient visits by the 91 patients who had clustered isolates revealed only one possible instance of patient-to-patient transmission.
The DNA fingerprinting of all M. tuberculosis isolates from a 1-year period revealed one possible instance of nosocomial transmission and five false-positive M. tuberculosis cultures. (...)
Mots-clés Pascal : Tuberculose, Mycobactériose, Bactériose, Infection, Epidémiologie, Enquête publique, DNA, Méthode fingerprint, Exploration bactériologique, Transmission homme homme, Analyse statistique, Evaluation, Mycobacterium tuberculosis, Mycobacteriaceae, Mycobacteriales, Actinomycetes, Bactérie, Homme, Etats Unis, Amérique du Nord, Amérique, Biologie moléculaire, Immunopathologie, Prévention
Mots-clés Pascal anglais : Tuberculosis, Mycobacterial infection, Bacteriosis, Infection, Epidemiology, Public inquiry, DNA, Fingerprint method, Bacteriological investigation, Transmission from man to man, Statistical analysis, Evaluation, Mycobacterium tuberculosis, Mycobacteriaceae, Mycobacteriales, Actinomycetes, Bacteria, Human, United States, North America, America, Molecular biology, Immunopathology, Prevention
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0019403
Code Inist : 002B05B02O. Création : 31/05/1999.