IgE antibodies specific for carbohydrates in a patient allergic to gum arabic (Acacia senegal).
The present study deals with the detailed investigation of the IgE antibody response of a gum arabic-allergic patient.
The patient showed multiple serologic and skin test sensitizations to a range of pollen, other inhalants and foods, and bee venom, and to the recombinant allergens Bet v 1 and Bet v 2. Moreover, the patient's serum reacted strongly to gum-arabic extract.
The NaIO4-treated and thus deglycosylated extract showed no binding to IgE.
In contrast, removal of the protein backbone by basic hydrolysis did not deplete the IgE reactivity.
Therefore, it is concluded that the gum arabic-specific IgE antibodies of this patient were mainly directed against the carbohydrate fraction of this material.
In IgE-inhibition assays, cross-reactions occurred in the range of 60% between gum arabic and known immunogenic N-glycans containing alpha1-3-linked fucose.
Since the inhibition graphs were not parallel and the inhibition was not complete with heterologue antigens, the cross-reacting epitopes of gum arabic appeared to be different from the latter well-known cross-reactive carbohydrate determinants (CCD).
Inhibition may have been caused by a partial immunologic identity of the investigated carbohydrate moieties.
A strong IgE response to the fucose-containing glycan from bromelain was measured in a glycan ELISA that utilizes purified glycopeptides at the solid phase. (...)
Mots-clés Pascal : Allergie, Gomme arabique, Acacia senegal, Leguminosae, Dicotyledones, Angiospermae, Spermatophyta, Allergène, Déterminant antigénique, Antigène carbohydrate, IgE, Exploration immunologique, Homme, Etude cas, Mâle, Adulte, Maladie professionnelle, Immunopathologie, Médecine travail
Mots-clés Pascal anglais : Allergy, Gum arabic, Acacia senegal, Leguminosae, Dicotyledones, Angiospermae, Spermatophyta, Allergen, Antigenic determinant, Carbohydrate antigen, IgE, Immunological investigation, Human, Case study, Male, Adult, Occupational disease, Immunopathology, Occupational medicine
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0006074
Code Inist : 002B06C05. Création : 31/05/1999.