Urinary 5-aminolevulinic acid (ALA) adjusted by creatinine : A surrogate for plasma ALA ?
5-Aminolevulinic acid (ALA) is the first intermediate substrate in the heme synthetic pathway and is the substrate of aminolevulinic acid dehydratase (ALAD, porphobilinogen synthase).
Because lead effectively inhibits ALAD activity, resulting in accumulation ofALA in urine and blood, urinary ALA (ALAU) has been used as a biomarker for lead exposure or early biologic effect of lead.
Intraindividual variation in urinary excretion of ALA requires the use of 24-hour urine samples or adjustment of single urine samples by other normalizing variables, such as urinary creatinine concentration.
Previous studies of ALAU concentration have used various adjustment methods ; however, few have compared creatinine-adjusted ALAU concentration with ALA concentration in plasma (ALAP) from subjects with low (<30 mug/dL) to moderate (<60 mug/dL) levels of blood lead.
To determine if creatinine-adjusted ALA U is associated with ALAP, we measured ALAU, ALAP, and urinary creatinine in 65 Korean lead workers with blood lead concentrations in the range of 14-60 mug/dL.
ALAU, ALAU/creatinine, or ALAU/log creatinine all correlated with ALAP.
However, ALAU/creatinine correlated more closely with ALAP based on Spearman's r (rs=0.40, P=0.0009), supporting the use of ALA/creatinine in single urine samples as a surrogate for ALAP.
Mots-clés Pascal : Plomb, Métal lourd, Exposition professionnelle, Médecine travail, Surveillance biologique, Marqueur biologique, Liquide biologique, Urine, Méthode étude, Sang, Créatinine, Industrie, Pile électrochimique, Fabrication, Homme, 5-aminolevulinique acide
Mots-clés Pascal anglais : Lead, Heavy metal, Occupational exposure, Occupational medicine, Biological monitoring, Biological marker, Biological fluid, Urine, Investigation method, Blood, Creatinine, Industry, Electrochemical cell, Manufacturing, Human
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 99-0001600
Code Inist : 002B03L05. Création : 31/05/1999.