The dust derived from an aluminium electrolytic plant was collected on a filter, then extracted by mixed solvent of benzene, hexane and isopropanol (7/2/1, v/v).
The solvent-soluble components was separated into five fractions, namely organic acids, organic alkalis, aliphatic hydrocarbons, polycyclic aromatic hydrocarbons and polar compounds.
The genotoxicities of the dust organic extract and its five fractions were examined with Ames test, unscheduled DNA systhesis (UDS), micronucleus (MN) and sister-chromatid exchange (SCE) tests in human lymphocytes in vitro which involve the different test systems (bacteria and mammalian cells) and three genetic endpoints (gene mutation, chromosome aberration and DNA damage).
The results of four experiments indicated that the dust organic extract showed higher genotoxicity.
Among the five fractions, three fractions, namely organic acids, polycyclic aromatic hydrocarbons and polar compounds had higher genotoxicity than others.
The other fractions, organic alkalis and aliphatic hydrocarbons had no genotoxicity.
According to this study, it is necessary to take effective measures to abate the dust and protect the environment and human health.
Mots-clés Pascal : Toxicité, DNA, In vitro, Poussière, Acide organique, Métal alcalin Composé organique, Hydrocarbure, Composé aliphatique, Composé aromatique polycyclique, Médecine travail, Exposition professionnelle, Usine aluminium, Test mutagénicité, Test Ames, Test micronucléus, Echange chromatide soeur, Synthèse DNA non programmée
Mots-clés Pascal anglais : Toxicity, DNA, In vitro, Dust, Organic acids, Alkali metal Organic compounds, Hydrocarbon, Aliphatic compound, Polycyclic aromatic compound, Occupational medicine, Occupational exposure, Aluminum plant, Mutagenicity testing, Ames test, Micronucleus test, Sister chromatid exchange, Unscheduled DNA synthesis
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 98-0509149
Code Inist : 002B03L03. Création : 23/03/1999.