To determine the prevalence and type of Y chromosome microdeletions in 136 consecutively seen intracytoplasmic sperm injection (ICSI) candidates and in 50 consecutively seen azoospermic men attending an infertility clinic.
Controlled clinical study.
Genetics laboratory and infertility clinic at a University hospital.
One hundred eighty-six men who were seen at an infertility clinic and who were referred to a genetics counseling service for genetic assessment before ICSI.
Collection of semen and blood samples.
Semen analysis ; serum FSH, LH, and T levels :
Semen analysis ;
karyotype analysis ;
and presence or absence of several single tagged site markers along the Y chromosome (sY274, sY238, sY276, sY84, sY102, sY143, sY153, sY254, sY269, sY202, sY158, sY160).
Yq chromosome microdeletions were detected in 10 (5.4%) of 186 consecutively seen ICSI candidates.
The number of microdeletions was much higher in azoospermic patients (16% ; 8 of 50) than in oligospermic patients (1.5% ; 2 of 136).
Two of the azoospermic patients with a Yq microdeletion also had sex chromosome aneuploidy mosaicism.
No microdeletions were detected in 100 consecutively seen fathers who were included as controls. (...)
Mots-clés Pascal : Injection spermatozoïde intracytoplasmique, Prévalence, Délétion, Chromosome Y, Chromosome Y anormal, Chromosome anormal, Aberration chromosomique, Azoospermie, Oligospermie, Procréation assistée, Epidémiologie, Chromosome sexuel, Chromosome sexuel anormal, Appareil génital mâle pathologie, Stérilité mâle, Sperme pathologie, Homme, Mâle
Mots-clés Pascal anglais : Intracytoplasmic sperm injection, Prevalence, Deletion, Y-Chromosome, Abnormal Y chromosome, Abnormal chromosome, Chromosomal aberration, Azoospermia, Oligospermia, Assisted procreation, Epidemiology, Sex chromosome, Abnormal sex chromosome, Male genital diseases, Male sterility, Semen disorders, Human, Male
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 98-0502945
Code Inist : 002B20A04. Création : 19/02/1999.