Benzene oxide (BO) reacts with cysteinyl residues in hemoglobin (Hb) and albumin (Alb) to form protein adducts (BO-Hb and BO-Alb), which are presumed to be specific biomarkers of exposure to benzene.
We analyzed BO-Hb in 43 exposed workers and 42 unexposed controls, and BO-Alb in a subsample consisting of 19 workers and 19 controls from Shanghai, China, as part of a larger cross-sectional study of benzene biomarkers.
The adducts were analyzed by gas chromatography-mass spectrometry following reaction of the protein with trifluoroacetic anhydride and methanesulfonic acid.
When subjects were divided into controls (n=42) and workers exposed to <=31 (n=21) and>31 p.p.m. (n=22) benzene, median BO-Hb levels were 32.0,46.7 and 129 pmol/g globin, respectively (correlation with exposure : Spearman r=0.67, P<0.0001).
To our knowledge, these results represent the first observation in humans that BO-Hb levels are significantly correlated with benzene exposure.
Median BO-Alb levels in these 3 groups were 103 (n=19), 351 (n=7) and 2010 (n=12) pmol/g Alb, respectively, also reflecting a significant correlation with exposure (Spearman r=0.90, P<0.0001).
The blood dose of BO predicted from both Hb and Alb adducts was very similar.
These results clearly affirm the use of both Hb and Alb adducts of BO as biomarkers of exposure to high levels of benzene. (...)
Mots-clés Pascal : Benzène, Toxicité, Dose forte, Exposition professionnelle, Médecine travail, Homme, Adduit moléculaire, Protéine, Albumine, Hémoglobine, Marqueur biologique, Marqueur tumoral, Benzène oxyde
Mots-clés Pascal anglais : Benzene, Toxicity, High dose, Occupational exposure, Occupational medicine, Human, Molecular adduct, Protein, Albumin, Hemoglobin, Biological marker, Tumoral marker
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 98-0490302
Code Inist : 002B03L06. Création : 19/02/1999.