Elevated frequency of sister chromatid exchanges in lymphocytes of victims of the Tokyo sarin disaster and in experiments exposing lymphocytes to by-products of sarin synthesis.
More than 5000 passengers of Tokyo subway trains were injured with toxic chemicals including the nerve gas sarin.
Most of the victims examined had marked miosis and decreased serum cholinesterase activity.
To monitor the genetic aftereffects of sarin exposure, we measured sister chromatid exchanges (SCEs) of the victims using peripheral blood lymphocytes.
The frequency of SCEs was significantly higher in the victims than in the control group.
Analyzing results using samples of urine from the victims suggested that the victims were exposed to not only sarin per se, but by-products of sarin synthesis. i.e. diisopropyl methylphosphonate (DIMP), diethyl methylphosphonate (DEMP) and ethyl isopropyl methylphosphonate (EIMP).
Thus, the in vitro SCE-inducing effect of DIMP, DEMP and EIMP was examined using human lymphocytes and we obtained positive results.
Mots-clés Pascal : Surveillance biologique, Test mutagénicité, Echange chromatide soeur, Lymphocyte, Homme, Attaque chimique, Organophosphoré, Phosphonate organique, Sous produit, Métabolite, Intoxication, Japon, Asie, Arme chimique, Neurotoxine, Anticholinestérasique, Station métro, Toxicité, Cytotoxicité, Sang, Hémopathie, In vitro, Mutagène, Toxicologie, Sarin
Mots-clés Pascal anglais : Biological monitoring, Mutagenicity testing, Sister chromatid exchange, Lymphocyte, Human, Chemical etching, Organophosphorus compounds, Organic phosphonate, By product, Metabolite, Poisoning, Japan, Asia, Chemical warfare agent, Neurotoxin, Anticholinesterase agent, Subway station, Toxicity, Cytotoxicity, Blood, Hemopathy, In vitro, Mutagen, Toxicology
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 98-0470726
Code Inist : 002B03L02. Création : 19/02/1999.