Stimulation of hydrogen peroxide production by drinking water contaminants in HL-60 cells sensitized by retinoic acid.
Chemical carcinogens, such as chloroform and trichloroethylene, are present in drinking water in Japan.
As these contaminants are believed to have a role in carcinogenesis, we examined if chloroform and trichloroethylene, as well as methylene chloride, xylene, benzene, and ethanol, have the ability to generate hydrogen peroxide (H2O2) in human polymorphonuclear leukocytes (PMN) and human leukemia (HL-60) cells.
Methylene chloride, benzene, xylene, trichloroethylene, and ethanol did not increase cellular H2O2 production as measured by flow cytometry nor as observed by confocal laser microscopy.
In PMN and RA-untreated HL-60 cells chloroform did not significantly affect H2O2 levels.
However, in HL-60 cells sensitized by pretreatment of 10 nM retinoic acid (RA) for 12 h, chloroform induced a significant increase in H2O2, but the increase induced by trichloroethylene was not significant.
The observed increase in fluorescence was confirmed using a confocal laser microscope.
These results indicate that chloroform and trichloroethylene may stimulate H2O2 production in HL-60 cells sensitized by pretreatment of RA.
Our method may be useful to test if weak stimulants can stimulate intracellular H2O2 production.
Mots-clés Pascal : Trichloroéthylène, Chloroforme, Rétinoïque acide, Hydrogène, Peroxyde, Eau alimentation, Pollution eau, Leucémie, Santé et environnement, Carcinogenèse, Homme, Cellule, In vitro, Japon, Asie, Toxicité, Hémopathie maligne
Mots-clés Pascal anglais : Trichloroethylene, Chloroform, Retinoic acid, Hydrogen, Peroxides, Feed water, Water pollution, Leukemia, Health and environment, Carcinogenesis, Human, Cell, In vitro, Japan, Asia, Toxicity, Malignant hemopathy
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 98-0441243
Code Inist : 002B04E02. Création : 25/01/1999.