Antigen specificity of antihistone antibodies in systemic sclerosis.
Objectives-The aim of the study was to determine the prevalence and clinical significance of antibodies to individual histone components in systemic sclerosis (SSc).
Methods-Serum samples from patients with limited cutaneous SSc (lSSc ; n=42) and diffuse cutaneous SSc (dSSc ; n=28) were examined for IgG and/or IgM antibodies to individual histone components and complexes by enzyme linked immunosorbent assay (ELISA).
The level of IgG antibody to total histones was significantly higher in lSSc and dSSc than in normal controls.
The level of IgM antibody to total histones was significantly higher in lSSc, but not in dSSc, than in normal controls.
IgG antibody to total histones tended to be increased in dSSc when compared with that in lSSc.
On the other hand, IgM antibody to total histones tended to be increased in lSSc when compared with that in dSSc.
Although SSc showed various antihistone specificities, H2B, H2A-H2B, (H2A-H2B) - dsDNA were main antigens recognised by IgG antibodies in both lSSc and dSSc.
Although IgM antibodies to H2B and H2A-H2B were also detected in both lSSc and dSSc, serum samples from lSSc patients exhibited highest IgM reactivity with H1.
Conclusion-SSc may be included among conditions in which heterogeneous antihistone antibodies are produced. (...)
Mots-clés Pascal : Sclérodermie, Symptomatologie, Analyse corrélation, Epidémiologie, Prévalence, Anticorps, Autoanticorps, Histone, Sérum, Technique ELISA, Spécificité, Immunoglobuline, IgG, IgM, Homme, Peau pathologie, Tissu conjonctif pathologie, Maladie système, Maladie autoimmune, Immunopathologie
Mots-clés Pascal anglais : Scleroderma, Symptomatology, Correlation analysis, Epidemiology, Prevalence, Antibody, Autoantibody, Histone, Serum, ELISA assay, Specificity, Immunoglobulins, IgG, IgM, Human, Skin disease, Connective tissue disease, Systemic disease, Autoimmune disease, Immunopathology
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 98-0432831
Code Inist : 002B07. Création : 25/01/1999.