Previous studies have shown a significant association between confirmed diisocyanate-induced asthma (DOA) and in vitro production of diisocyanate antigen-stimulated histamine-releasing factors by PBMCs.
Chemokines found in PBMC supernatants are known to express histamine-releasing factor activity.
PBMCs of diisocyanate-exposed workers were tested in vitro for diisocyanate antigen-specific enhancement of monocyte chemoattractant protein-1 (MCP-1), monocyte chemoattractant protein-3 (MCP-3), macrophage inflammatory protein-1alpha, RANTES, IL-8, and T-cell cytokines that could play a regulatory role in chemokine synthesis (IL-4, IL-5, IFN-gamma, and TNF-alpha).
Secretion of chemokines and cytokines was determined by quantitative immunochemical assays of PBMC supernatants.
Synthesis of mRNA for bêta-chemokines was determined by reverse transcription-polymera : chain reaction.
PBMCs of workers with DOA showed significantly enhanced secretion for MCP-I compared with diisocyanate-exposed asymptomatic workers (P<. 05).
In vitro induction of antigen-stimulated MCP-1 mRNA synthesis in cultured PBMCs was demonstrated by reverse-transcription polymerase chain reaction.
Quantitation of cytokines in supernatants showed increased mean production of IL-8 and TNF-a.
IFN-gamma, IL-4, and IL-5 were not enhanced in subjects with DOA. (...)
Mots-clés Pascal : Asthme, Exposition professionnelle, Pathogénie, Diisocyanate organique, Inflammation, Voie respiratoire, Chimiotactisme, Mononucléaire, Macrophage, Facteur nécrose tumorale alpha, Interleukine 8, Homme, Appareil respiratoire pathologie, Bronchopneumopathie obstructive, Médecine travail, Chimiokine, Cytokine, Protéine chimiotactique monocyte 1
Mots-clés Pascal anglais : Asthma, Occupational exposure, Pathogenesis, Organic diisocyanate, Inflammation, Respiratory tract, Chemotaxis, Mononuclear cell, Macrophage, Tumor necrosis factor alpha, Interleukin 8, Human, Respiratory disease, Obstructive pulmonary disease, Occupational medicine, Chemokine, Cytokine, Monocyte chemoattractant protein 1
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 98-0425063
Code Inist : 002B11B. Création : 25/01/1999.