The genotype of hepatitis C virus (HCV) of 172 HCV-RNA positive serum specimens taken from patients with chronic liver diseases. thalassaemia major, chronic renal failure (CRF). haemophilia and intravenous drug abusers (IVDA) was determined by analysis of the amplified 5'UTR region by genotype-specific oligonucleotide probes and restriction fragment length polymorphism (RFLP).
Six different genotypes and subtypes (1a, 1b, 2,3,4 and 6) were found.
Genotype 1b was the predominant genotype among patients with chronic liver diseases (69.6%). followed by genotype 6 (18.8%), which was similar to that reported for blood donors in earlier studies.
Pronounced differences in the distribution of genotypes were seen between the four risk groups.
Patients with CRF had a similar distribution to those with chronic liver diseases, whilst the greatest diversity of genotypes was seen in patients with haemophilia, which was expected since they were given factor VIII manufactured overseas.
Genotype 6 was particularly prominent in patients with thalassaemia major (50%) and IVDA (62.5%). It is possible that clonal spread of HCV genotype 6 has taken place among a closed subset of the population in Hong Kong through intravenous drug abuse.
Mots-clés Pascal : Hépatite virale C, Virose, Infection, Génotype, Risque, Réaction chaîne polymérase, Polymorphisme longueur fragment restriction, Epidémiologie, Prévalence, Homme, Hong Kong, Chine, Asie, Appareil digestif pathologie, Foie pathologie, Biologie moléculaire, Groupe population
Mots-clés Pascal anglais : Viral hepatitis C, Viral disease, Infection, Genotype, Risk, Polymerase chain reaction, Restriction fragment length polymorphism, Epidemiology, Prevalence, Human, Hong Kong, China, Asia, Digestive diseases, Hepatic disease, Molecular biology
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 98-0413273
Code Inist : 002B05C02G. Création : 25/01/1999.