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  1. Frequent occurrence of anti-tRNAHis autoantibodies that recognize a conformational epitope in sera of patients with myositis.

    Article - En anglais

    Objective

    To investigate the incidence of autoantibodies directed to deproteinized transfer RNAHis (tRNAHis) in anti-Jo-1 positive myositis patients and to determine the major B cell epitope.

    Methods

    One hundred sixty-seven myositis sera were screened by immunoblotting and enzyme-linked immunosorbent assay for the presence of anti-Jo-1 antibody.

    Autoantibodies directed to deproteinized RNA were detected by immunoprecipitation.

    Ribonuclease cleavage experiments were performed to determine the tRNAHis-specific features important for recognition.

    Results

    Approximately one-third of the anti-Jo-1 positive sera also contained autoantibodies recognizing tRNAHis.

    This recognition was independent of modified bases, but the presence of stabilizing Mg2+ions appeared to be essential for efficient immunoprecipitation.

    Transfer RNAHis-specific features in the anticodon loop were not protected from ribonuclease cleavage by bound antibodies, while protection of bases located in the D and T loops was observed.

    Conclusion

    A significant number of anti-Jo-1 positive myositis sera contain anti-tRNAHis activity.

    Formation of the major autoepitope on tRNAHis is strongly dependent on proper folding of this molecule mediated by an interaction between D and T loops which is stabilized by either modified residues or Mg2+ions.

    Mots-clés Pascal : Polymyosite, Idiopathique, Inflammation, Epidémiologie, Incidence, Lymphocyte B, Anticorps, Autoanticorps, Déterminant antigénique, Technique ELISA, Sérum, RNA transfert, Homme, Immunoprécipitation, In vitro, Muscle strié pathologie, Maladie système, Antigène Jo-1

    Mots-clés Pascal anglais : Polymyositis, Idiopathic, Inflammation, Epidemiology, Incidence, B-Lymphocyte, Antibody, Autoantibody, Antigenic determinant, ELISA assay, Serum, tRNA, Human, Immunoprecipitation reaction, In vitro, Striated muscle disease, Systemic disease

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 98-0409987

    Code Inist : 002B07. Création : 25/01/1999.