In western countries, the transmission of human herpesvirus 8 (HHV-8) via blood transfusion has been recently postulated.
In sub-Saharan African, the incidence of HHV-8-associated Kaposi's sarcoma and the seroprevalence for HHV-8 in autochthonous populations are high.
The aim of this study was to estimate the prevalence of blood donations potentially infectious for HHV-8 in the general adult population of Central Africa.
Forty-nine blood donors at the Centre de Transfusion Sanguine in Bangui, the capital of the Central African Republic, were selected.
Forty-five inpatients of Broussais Hospital, Paris, France, who were known to be seronegative for HIV and hepatitis B and C viruses and who had not received heart or kidney transplants, were chosen as a European « control » group for comparison.
HHV-8 DNA sequences were detected in peripheral blood mononuclear cells by nested polymerase chain reaction using primer sets located in the HHV-8 open reading frame 26.
Eleven (22.5% ; 95% Cl : 12% - 37%) of 49 blood donors were positive for HHV-8.
Three (6%) were HIV-1 seropositive.
Two (67%) of the 3 HIV-infected blood donors were also positive for HHV-8.
All blood donors were apparently healthy ; none was known to suffer from Kaposi's sarcoma.
Only one (2.2%) control was carrying HHV-8 DNA on the peripheral blood mononuclear cells. (...)
Mots-clés Pascal : Don sang, Afrique Centrale, Afrique, Donneur sang, Risque infectieux, Porteur, Asymptomatique, Réaction chaîne polymérase, Prévalence, Epidémiologie, Adulte, Homme, Biologie moléculaire, Herpesvirus hominis 8
Mots-clés Pascal anglais : Blood donation, Central Africa, Africa, Blood donor, Infectious risk, Carrier, Asymptomatic, Polymerase chain reaction, Prevalence, Epidemiology, Adult, Human, Molecular biology
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 98-0409495
Code Inist : 002B27D01. Création : 25/01/1999.