Molecular methods for the diagnosis of genital ulcer disease in a sexually transmitted disease clinic population in Northern Thailand : Predominance of herpes simplex virus infection.
A multiplex polymerase chain reaction (M-PCR) assay that simultaneously detects the three major causes of genital ulcer disease (GUD), Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus, was used to evaluate swab specimens for 38 sequential patients with GUD at a Thai sexually transmitted disease clinic.
Subjects received clinical diagnoses and syndromic treatment.
Swab specimens for H. ducreyi cultures and M-PCR were obtained.
No H. ducreyi cultures were positive.
Of 38 M-PCR specimens, 31 (81.6%) were positive for HSV, 1 (2.3%) for both HSV and T. pallidum, and none for H. ducreyi or T. pallidum alone ; 6 (15.8%) were negative for all 3 pathogens.
Clinical diagnoses corresponded poorly to M-PCR findings ; none of 5 suspected cases of chancroid were positive by M-PCR and none of 1 for syphilis, but 21 of 24 suspected herpes lesions were confirmed by M-PCR.
Human immunodeficiency virus infection status was known for 24 of 38 subjects ; 11 (45.8%) were seropositive, and all 11 had HSV by M-PCR.
HSV appeared to be the most common pathogen overall.
Mots-clés Pascal : Herpesvirus hominis, Alphaherpesvirinae, Herpesviridae, Virus, Treponema pallidum, Treponemataceae, Spirochaetales, Bactérie, Haemophilus ducreyi, Pasteurellaceae, Diagnostic, Prélèvement, Maladie sexuellement transmissible, Identification, Epidémiologie moléculaire, Prévalence, Etiologie, Ulcération, Appareil génital pathologie, Thaïlande, Asie
Mots-clés Pascal anglais : Herpesvirus hominis, Alphaherpesvirinae, Herpesviridae, Virus, Treponema pallidum, Treponemataceae, Spirochaetales, Bacteria, Haemophilus ducreyi, Pasteurellaceae, Diagnosis, Samplings, Sexually transmitted disease, Identification, Molecular epidemiology, Prevalence, Etiology, Ulceration, Genital diseases, Thailand, Asia
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 98-0408267
Code Inist : 002B05C02E. Création : 25/01/1999.