Background-Infection with human parvovirus B19 (B19) has been reported in a few patients with various vasculitis syndromes.
Mixed cryoglobulinaemia (MC), a model of small vessel size vasculitis, may result from numerous infectious diseases, particularly hepatitis C virus (HCV) infection.
Aim-To assess the prevalence of seric B19 infection markers in a large series of patients with MC, with or without HCV infection.
Patients and methods-Sixty four patients were studied : essential MC (EMC, n=19), MC associated with non-infectious diseases (non-essential MC, n=9), and patients with HCV infection with (HCV-MC, n=18) or without MC (HCV-no-MC, n=18).
Patients were considered to have MC if two successive determinations of their serum cryoglobulin concentration were above 0.05 gfl.
Serum samples were analysed for specific IgG and IgM antibodies to B19 by enzyme immunoassay.
B19 DNA detection was performed by polymerase chain reaction using a set of primers located in the VP1 gene, separately in serum and in cryoprecipitates to investigate a possible capture of B19 DNA in cryoprecipitate.
The study also looked for a possible enrichment for of IgG antibodies to B19 in MC.
The presence of specific IgG antibodies to B19 was found in 68% EMC, 56% non-essential MC, 78% HCV-MC, and 78% HCV-no-MC.
No patient of either group had specific IgM antibodies to B19, or B19 DNA in serum or in cryoprecipitate. (...)
Mots-clés Pascal : Cryoglobulinémie mixte, Association, Homme, Virus B19, Erythrovirus, Parvovirinae, Parvoviridae, Virus, Infection, Symptomatologie, Hépatite virale C, Virose, Marqueur biologique, Sérum, Epidémiologie, Prévalence, Vaisseau sanguin pathologie, Vascularite, Maladie système, Immunopathologie, Appareil digestif pathologie, Foie pathologie
Mots-clés Pascal anglais : Mixed cryoglobulinemia, Association, Human, Virus B19, Erythrovirus, Parvovirinae, Parvoviridae, Virus, Infection, Symptomatology, Viral hepatitis C, Viral disease, Biological marker, Serum, Epidemiology, Prevalence, Vascular disease, Vasculitis, Systemic disease, Immunopathology, Digestive diseases, Hepatic disease
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 98-0406337
Code Inist : 002B07. Création : 25/01/1999.