T-lymphocyte responses to plicatic acid-human serum albumin conjugate in occupational asthma caused by western red cedar.
T cells are known to play a major role in the pathogenesis of atopic allergic asthma, but it is less clear whether they are involved in occupational asthma caused by low molecular weight chemicals such as plicatic acid.
We sought to determine whether peripheral blood T cells from patients with western red cedar asthma (WRCA) recognize plicatic acid (PA) conjugated to human serum albumin (HSA) as judged by proliferation or cytokine production and to analyze the response to PA inhalation with flow cytometry.
Significant proliferative responses to PA-HSA were observed in eight of 33 patients with WRCA, none of 10 exposed nonasthmatic cedar workers, and one of 18 nonasthmatic control subjects.
Two of 25 patients with WRCA also showed proliferative responses to unconjugated PA.
All the WRCA responders were either currently exposed to cedar or had ceased exposure within the preceding 2 years.
None of the four patients receiving oral steroids responded, but inhaled steroids did not seem to influence responsiveness.
No correlations were found between the maximum stimulation response and any of the current FEV1 values, the current PC20 methacholine values, or the magnitude of the late asthmatic response to PA.
Peripheral blood T-cell subset proportions and their degree of activation were similar in patients with WRCA and exposed control subjects. (...)
Mots-clés Pascal : Asthme, Thuja plicata, Coniferales, Gymnospermae, Spermatophyta, Exposition professionnelle, Lymphocyte T, Immunité cellulaire, Réponse immune, Physiopathologie, Interféron gamma, Interleukine 5, Allergie, Allergène, Arbre, Canada, Amérique du Nord, Amérique, Homme, Appareil respiratoire pathologie, Bronchopneumopathie obstructive, Immunopathologie, Médecine travail, Maladie professionnelle, Cèdre rouge
Mots-clés Pascal anglais : Asthma, Thuja plicata, Coniferales, Gymnospermae, Spermatophyta, Occupational exposure, T-Lymphocyte, Cellular immunity, Immune response, Pathophysiology, Gamma interferon, Interleukin 5, Allergy, Allergen, Tree, Canada, North America, America, Human, Respiratory disease, Obstructive pulmonary disease, Immunopathology, Occupational medicine, Occupational disease
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 98-0383935
Code Inist : 002B06C02. Création : 25/01/1999.