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  1. Epidemiological study of p53 tumor suppressor gene mutations in patients from Luxembourg and the German Saar region with an advanced colorectal cancer using PCR-SSCP analysis.

    Article - En anglais

    Mutations in the p53 tumor suppressor gene are usually associated with an advanced development of colorectal cancer characterized by the transition from the adenoma to the carcinoma stage.

    We used the polymerase chain reaction (PCR) followed by single-strand conformation polymorphism (SSCP) analysis to screen for the presence of mutations in the p53 gene of patients from Luxembourg and the German Saar region with colorectal cancers at various developmental stages.

    While we detected no mutations in 16 colic polypi at an early to intermediate stage (adenoma), we revealed seven (13.7%) non-silent point mutations (transitions) in exons 5 to 9 of the p53 gene in 51 colorectal tumors at a late stage (carcinoma).

    In addition to confirming previous observations. these results show that PCR-SSCP analysis can provide both a sensitive and rapid method for the genetic determination of the histopathological stage of colorectal samples.

    Mots-clés Pascal : Polype adénomateux, Tumeur maligne, Côlon, Rectum, Homme, Gène TP53, Gène suppresseur tumeur, Mutation somatique, Stade avancé, Luxembourg, Europe, Sarre, Allemagne, Epidémiologie, Grade histologique, Appareil digestif pathologie, Intestin pathologie, Côlon pathologie, Rectum pathologie, Génétique

    Mots-clés Pascal anglais : Adenomatous polyp, Malignant tumor, Colon, Rectum, Human, TP53 Gene, Tumor suppressor gene, Somatic mutation, Advanced stage, Luxembourg, Europe, Saarland, Germany, Epidemiology, Histological grading, Digestive diseases, Intestinal disease, Colonic disease, Rectal disease, Genetics

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 98-0383921

    Code Inist : 002B13B01. Création : 25/01/1999.