Analysis of fumonisin B1 and its hydrolysis product in Tortillas.
Fumonisins are toxic metabolites of Fusarium moniliforme, a fungus that occurs widely in corn.
Fumonisins cause leukoencephalomalacia in horses and pulmonary edema in swine and have been suggested as a possible cause of an increased incidence of neural tube defects among people living along the Texas-Mexico border.
As part of an effort to determine levels of fumonisins in human food, a liquid chromatographic (LC) method was devised for determining fumonisin B1 (FB1) and the total hydrolysis product of FB1 (HB1) in tortillas.
The method uses acetonitrile-0.1 M phosphate buffer (pH 3 ; 1+1) extraction, solid-phase C18 cleanup, o-phthalaldehyde and 2-mercaptoethanol derivatization, and reversed-phase LC.
Average recoveries from tortillas spiked with FB1 and HB1 at 250,500, and 1000 ng/g were 86.5% for FB1 and 82.6% for HB1 Tortillas (54) and masa (8) from the Texas-Mexico border were analyzed for FB1 and HB1 Average amounts of FB1 and HB1 in tortillas were 187 and 82 ng/g, respectively.
Average amounts of FB1 and HB1 in masas were 262 and 64 ng/g, respectively.
The results show that fumonisin B1 and its hydrolysis product are present in tortillas consumed by a population experiencing an increased incidence of neural tube defects.
Mots-clés Pascal : Contrôle qualité, Extraction SPE, Chromatographie phase inverse, Dérivatisation, Frontière, Tortilla, Contamination, Fumonisine, Maïs, Méthode analyse, Analyse quantitative, Produit céréalier, Mycotoxine, Toxine, Texas, Etats Unis, Amérique du Nord, Amérique, Chromatographie phase liquide, Mexique, Amérique Centrale, Homme
Mots-clés Pascal anglais : Quality control, Solid phase extraction, Reversed phase chromatography, Derivatization, Border, Tortilla, Contamination, Fumonisin, Corn, Analysis method, Quantitative analysis, Cereal product, Mycotoxin, Toxin, Texas, United States, North America, America, Liquid chromatography, Mexico, Central America, Human
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 98-0355761
Code Inist : 002A35B03. Création : 25/01/1999.