Pulmonary tuberculosis in Harare, Zimbabwe : analysis by spoligotyping.
Background-Over the last 10 years there has been a fourfold increase in cases of tuberculosis in Harare, Zimbabwe.
The use of molecular epidemiology to understand tuberculosis transmission in this epidemic has been hampered by the availability of suitable culture facilities.
A study was therefore undertaken to explore the potential of spoligotyping, a polymerase chain reaction based technique that does not require tuberculosis culture.
Methods-Adults attending a chest clinic with clinical or radiological pulmonary tuberculosis and one smear positive sputum were enrolled over one month.
Demographic, socioeconomic, and clinical data were gathered using a standardised questionnaire.
Molecular fingerprinting of genomic DNA recovered from sputum was performed by spoligotyping.
Sixty one subjects (median age 28 years (range 18-73) ; 61% men) were recruited and 57 provided adequate sputum samples.
Recent rural-urban migration or immigration was not common ; 40% of subjects lived in crowded living conditions.
DNA suitable for spoligotyping was recovered from 28 patients and 20 different genotypes of Mycobacterium tuberculosis were identified.
Fifteen patients were infected with an M tuberculosis strain shared by one or more individuals.
Patients infected with a shared spoligotype were not closely linked geographically within Harare, but were more likely to live in overcrowded conditions (69% versus 23% ; odds ratio 6.85 (95% CI 1.2 to 47), p=0.026). (...)
Mots-clés Pascal : Tuberculose, Mycobactériose, Bactériose, Infection, Poumon, SIDA, Virose, Infection opportuniste, Empreinte génomique, DNA, Zimbabwe, Afrique, Exploration, Epidémiologie, Homme, Appareil respiratoire pathologie, Poumon pathologie, Immunopathologie, Immunodéficit
Mots-clés Pascal anglais : Tuberculosis, Mycobacterial infection, Bacteriosis, Infection, Lung, AIDS, Viral disease, Opportunistic infection, Genomic imprinting, DNA, Zimbabwe, Africa, Exploration, Epidemiology, Human, Respiratory disease, Lung disease, Immunopathology, Immune deficiency
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 98-0295809
Code Inist : 002B05B02E. Création : 27/11/1998.