logo BDSP

Base documentaire

  1. Isolation and characterization of verocytotoxin-producing Escherichia coli O157 strains from Dutch cattle and sheep.

    Article - En anglais

    In the periods from July to November 1995 and 1996, fecal samples from Dutch cattle and sheep were collected at the main slaughterhouses of The Netherlands, located at different geographic sites.

    The samples were examined for the presence of verocytotoxin (VT) - producing Escherichia coli (VTEC) of serogroup 0157.

    E. coli O157 strains could be isolated from 57 (10.6%) of 540 adult cattle, 2 (0.5%) of 397 veal calves, 2 (3.8%) of 52 ewes, and 2 (4.1%) of 49 lambs.

    Immunomagnetic separation with 0157-specific-antibody-coated beads appeared to be significantly more sensitive than conventional plating for detection of the organism in feces.

    With the exception of two isolates from adult cattle which appeared to be negative for VT genes, all animal isolates were positive for both VT (VT1 and/or VT2) and E. coli attaching-and-effacing gene sequences, and therefore, they were regarded as potential human pathogens.

    Although genomic typing by pulsed-field gel electrophoresis revealed a wide variety of distinct restriction patterns, comparison of the 63 animal isolates with 33 fecal 0157 VTEC strains previously isolated from humans with the diarrhea-associated form of the hemolytic-uremic syndrome by their phage types and VT genotypes showed a marked similarity between animal and human isolates : 30 (90.9%) of the 33 human isolates appeared to be of E. coli O157 strain types also isolated from cattle and sheep. (...)

    Mots-clés Pascal : Escherichia coli, Enterobacteriaceae, Bactérie, Colibacillose, Bactériose, Infection, Bovin, Artiodactyla, Ungulata, Mammalia, Vertebrata, Mouton, Epidémiologie, Prévalence, Fèces, Zoonose, Transmission animal homme, Facteur risque, Toxine, Vétérinaire, Pays Bas, Europe, Toxine type Shiga

    Mots-clés Pascal anglais : Escherichia coli, Enterobacteriaceae, Bacteria, Colibacillosis, Bacteriosis, Infection, Bovine, Artiodactyla, Ungulata, Mammalia, Vertebrata, Sheep, Epidemiology, Prevalence, Feces, Zoonosis, Transmission from animal to man, Risk factor, Toxin, Veterinary, Netherlands, Europe, Shiga-like toxin

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 98-0264654

    Code Inist : 002A05B11. Création : 11/09/1998.