Annual Meeting of the American Pediatric Surgical Association. Naples, FL, USA, 1997/05/18.
Transplantation of fetal liver hematopoietic stem cells (HSCs) in utero has the potential to treat a variety of hematologic, immunologic, and metabolic diseases.
One prerequisite for broad clinical application is the establishment of a bank of fetal liver HSC tissue.
The authors describe their methods for processing fetal liver free of known human pathogens while maximizing HSC activity after cryopreservation.
The authors developed a protocol that separates the abortion decision from the donation decision and preserves confidentiality between donor and recipient.
Human fetal livers (12 to 14 weeks'gestation) were procured from aborted specimens and the light-density hematopoietic cells isolated by density centrifugation.
Total viable cell count increased with gestational age and averaged from 4.36 x 107 cells for 12-week livers to 2.0 x 108 cells for 14-week livers.
Flow cytometric analysis demonstrated the presence of early progenitors in fresh and thawed specimens and a low number of T cells in each group.
The functional capacity of fetal liver progenitors was assessed with colony-forming assays before and after cryopreservation.
Thawed specimens showed an average 63% recovery rate for the high-proliferative potential colony-forming cells, a primitive subset of progenitors thought to include HSC.
However, the more mature fraction of low-proliferative potential colony-forming cells had a recovery rate of only 35%. (...)
Mots-clés Pascal : Transplantation, Foie, In utero, Biologie, Ethique, Banque organe, Greffe, Cellule hématopoïétique, Foetus, Chirurgie
Mots-clés Pascal anglais : Transplantation, Liver, In utero, Biology, Ethics, Organ bank, Graft, Hematopoietic cell, Fetus, Surgery
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Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 98-0261033
Code Inist : 002B30A09. Création : 11/09/1998.