Comparison of free-living amoebae in hot water systems of hospitals with isolates from moist sanitary areas by identifying genera and determining temperature tolerance.
Legionella-contaminated hot water systems and moist sanitary areas in six hospitals were sampled for amoebae by following a standardized collection protocol.
Genus identifications and temperature tolerance determinations were made.
Amoebae identified as Hartmannella vermiformis (65%), Echinamoebae spp. (15%), Saccamoebae spp. (12%), and Vahlkampfia spp. (9%) were detected in 29 of 56 (52%) hot water samples.
Twenty-three of 49 (47%) swabs obtained from moist areas were amoeba positive.
The following genera were identified :
Hartmannella (15%), and Vanella (7%). The temperature tolerance of amoebae from hot water systems was strikingly different from that of amoebae from moist areas.
At 44°C on agar, 59% of amoebic isolates sampled from hot water systems showed growth.
The corresponding value for isolates from moist areas was only 17%. Six Acanthamoeba isolates from the moist areas were considered potential pathogens.
Four Hartmannella and two Saccamoeba isolates from hot water could be cultured at 53°C.
Mots-clés Pascal : Hôpital, Réseau distribution, Contamination biologique, Amoeba, Heterolobosea, Protozoa, Isolat, Identification, Hygiène, Etude comparative, Tolérance, Température, Eau chaude
Mots-clés Pascal anglais : Hospital, Distribution network, Biological contamination, Amoeba, Heterolobosea, Protozoa, Isolate, Identification, Hygiene, Comparative study, Tolerance, Temperature, Hot water
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 98-0259177
Code Inist : 002B30A04A. Création : 11/09/1998.