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  1. Random amplification of polymorphic DNA (RAPD), pulsed-field gel electrophoresis (PFGE) and phage-typing in the analysis of a hospital outbreak of Salmonella Enteritidis.

    Article - En anglais

    Isolates of Salmonella Enteritidis from 81 patients from Herlev Hospital or from Copenhagen County were analysed by random amplification of polymorphic DNA (RAPD), pulsed-field gel electrophoresis (PFGE) and phage-typing.

    Fourteen polymorphic markers from five decamer primers unambiguously placed all isolates into six RAPD groups : 65 isolates of phage-type 6 (PFGE type I) were resolved into three RAPD groups constituting 86,12, and 2%, respectively.

    A fourth RAPD group of 10 isolates was coincident with phage type 8 (PFGE type II) and two isolates, one phage-type 1, the other phage-type 4 (both PFGE type I) formed the fifth group.

    The sixth group of four isolates was not phage typeable and was PFGE type III.

    Forty outbreak-related isolates of phage-type 6 were resolved into three strains.

    No diversity of phage-type 6 was found among isolates unrelated to the outbreak.

    It is concluded that RAPD is useful as a tool in investigations of microbial outbreaks in its own right, or to supplement phage-typing and PFGE of Salmonella Enteritidis.

    Mots-clés Pascal : Salmonella enteritidis, Enterobacteriaceae, Bactérie, Bactériose, Infection, Milieu hospitalier, Marqueur RAPD, Electrophorèse champ pulsé, Bactériophage, Virus, Typage, Epidémiologie, Technique, Identification, Evaluation performance, Homme, Danemark, Europe

    Mots-clés Pascal anglais : Salmonella enteritidis, Enterobacteriaceae, Bacteria, Bacteriosis, Infection, Hospital environment, Random amplified polymorphic DNA, Pulsed field electrophoresis, Phage, Virus, Typing, Epidemiology, Technique, Identification, Performance evaluation, Human, Denmark, Europe

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 98-0251642

    Code Inist : 002B05B02F. Création : 11/09/1998.