Random amplification of polymorphic DNA (RAPD), pulsed-field gel electrophoresis (PFGE) and phage-typing in the analysis of a hospital outbreak of Salmonella Enteritidis.
Isolates of Salmonella Enteritidis from 81 patients from Herlev Hospital or from Copenhagen County were analysed by random amplification of polymorphic DNA (RAPD), pulsed-field gel electrophoresis (PFGE) and phage-typing.
Fourteen polymorphic markers from five decamer primers unambiguously placed all isolates into six RAPD groups : 65 isolates of phage-type 6 (PFGE type I) were resolved into three RAPD groups constituting 86,12, and 2%, respectively.
A fourth RAPD group of 10 isolates was coincident with phage type 8 (PFGE type II) and two isolates, one phage-type 1, the other phage-type 4 (both PFGE type I) formed the fifth group.
The sixth group of four isolates was not phage typeable and was PFGE type III.
Forty outbreak-related isolates of phage-type 6 were resolved into three strains.
No diversity of phage-type 6 was found among isolates unrelated to the outbreak.
It is concluded that RAPD is useful as a tool in investigations of microbial outbreaks in its own right, or to supplement phage-typing and PFGE of Salmonella Enteritidis.
Mots-clés Pascal : Salmonella enteritidis, Enterobacteriaceae, Bactérie, Bactériose, Infection, Milieu hospitalier, Marqueur RAPD, Electrophorèse champ pulsé, Bactériophage, Virus, Typage, Epidémiologie, Technique, Identification, Evaluation performance, Homme, Danemark, Europe
Mots-clés Pascal anglais : Salmonella enteritidis, Enterobacteriaceae, Bacteria, Bacteriosis, Infection, Hospital environment, Random amplified polymorphic DNA, Pulsed field electrophoresis, Phage, Virus, Typing, Epidemiology, Technique, Identification, Performance evaluation, Human, Denmark, Europe
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 98-0251642
Code Inist : 002B05B02F. Création : 11/09/1998.