The frequency of chromosomal aberrations (CA) and micronuclei (MN) in peripheral blood lymphocytes was compared in two groups of persons occupationally exposed to tritium either through the use of luminous paints or in the weapons industry, with the aim of finding a correlation between the CA and MN assays, the extent of radiation hazard, and the duration of occupational exposure to chemical forms of tritium and the ability of the latter to induce hazards in human lymphocytes.
CA analysis and the cytochalasin B micronucleus test were performed.
Urinary tritium in both groups was measured using a liquid scintillation method.
For purposes of comparison, 2 control groups (40 persons) were examined ; 24 exposed individuals were matched to their controls according to age, sex, and smoking habits.
Values recorded for the frequency of CA in the group of workers handling tritium in luminous dial painting were 0.015 ± 0.014 dicentrics/cell, 0.023 ± 0.017 total unstable CA/cell, and 0.035 ± 0.019 MN/binucleated cell.
Urinary levels of tritium in this group of employees ranged from 1.35 to 9.43 M Bq/l. As compared with their matched controls, in these workers the differences in the yield of CA as well as in MN were statistically highly significant (P<0.005 and P<0.003, respectively).
The same parameters analyzed in workers employed in the armament industry gave values of 0.001 ± 0.003 dicentrics/cell, 0.010 ± 0.008 total unstable CA/cell, and 0. (...)
Mots-clés Pascal : Tritium, Radioisotope, Toxicité, Homme, Exposition professionnelle, Médecine travail, Radiolésion, Irradiation, Dose faible, Cassure chromosomique, Aberration chromosomique, Test micronucléus, DNA, Lymphocyte
Mots-clés Pascal anglais : Tritium, Radioisotope, Toxicity, Human, Occupational exposure, Occupational medicine, Radiation injury, Irradiation, Low dose, Chromosome break, Chromosomal aberration, Micronucleus test, DNA, Lymphocyte
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 98-0247583
Code Inist : 002A08F02. Création : 11/09/1998.