Exposure to zinc fume may cause metal fume fever, an acute reaction characterized by an invasion ofneutrophils into the airways.
This investigation was conducted to examine the possibility that Zn2+and ZnO might stimulate the formation of oxygen radicals by human neutrophils.
Luminol-amplified chemiluminescence (CL) was monitored during 2 h from human neutrophils exposed to Zn2+or ZnO.
The response was compared to that of other metal ions and to that ofendotoxin and phorbol myristate acetate (PMA).
Zn2+ (6-50 muM) gradually caused a 2-6-fold increase of CL that reached an optimum after 70-80 min.
By contrast, Cd2+Cr2+Cr3+Fe2+Fe3+Ni2+or Co2+in corresponding concentrations did not increase the CL.
Similar to Zn2+endotoxin (40-640 mug/ml) caused a 2-5-fold increase of CL with an optimum after 70 min, and endotoxin (40 mug/ml) together with Zn2+ (50 muM) synergistically increased the CL.
ZnO (12 - 100 mug/ml) also augmented CL, with a 1.5 - 5-fold increase at 25 - 100 mug/ml ZnO but with a time response similar to that found after PMA stimulation, in which CL peaked after 20-40 min incubation.
Both Zn2+-and ZnO-induced CL was inhibited by manoalide, a phospholipase A2 inhibitor, with IC50 of 0.25 muM and 0.66 muM respectively.
These results indicate that Zn2+and ZnO both stimulates oxygen radical formation in human neutrophils and that this might contribute to the pathogenesis of zinc fume fever.
Mots-clés Pascal : Zinc, Métal, Toxicité, Fièvre vapeur métallique, Zinc Oxyde, Mécanisme action, Radical libre, Homme, Neutrophile, Pathogénie, In vitro, Exposition professionnelle, Médecine travail, Inhalation, Appareil respiratoire pathologie
Mots-clés Pascal anglais : Zinc, Metal, Toxicity, Metal fumes fever, Zinc Oxides, Mechanism of action, Free radical, Human, Neutrophil, Pathogenesis, In vitro, Occupational exposure, Occupational medicine, Inhalation, Respiratory disease
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 98-0148641
Code Inist : 002B03L02. Création : 21/07/1998.