The ability of seventy clinical laboratories in nine European countries to detect glycopeptide resistance in Gram-positive bacteria was investigated.
Results of routine tests were compared with those on the same strains by a reference method in national co-ordinating laboratories.
In addition, control strains were tested by some of the participants.
Errors in reporting susceptibility of Staphylococcus aureus to teicoplanin and vancomycin and coagulase-negative staphylococci to vancomycin were<1%. With coagulase-negative staphylococci however, 44 (3.4%) teicoplanin susceptible isolates were reported intermediate and six (0.4%) resistant ; 18 (58.1%) of 31 teicoplanin intermediate isolates were reported susceptible and five (16.1%) resistant ; and six of nine teicoplanin resistant isolates were reported susceptible and two intermediate.
All seven isolates of enterococci intermediate to vancomycin were reported susceptible.
Distribution of a known vancomycin intermediate strain of E. gallinarum indicated problems with vancomycin susceptibility testing (44.4% reported susceptible, 32.7% intermediate, 32.1% resistant) and identification (only 34.1% correct) of this organism.
Two of 28 teicoplanin resistant enterococci and three of 30 vancomycin resistant isolates were reported susceptible.
Among other organisms, one resistant Lactobacillus sp. was reported susceptible to teicoplanin and vancomycin. (...)
Mots-clés Pascal : Vancomycine, Teïcoplanine, Antibiotique, Antibactérien, Sensibilité résistance, Test sensibilité médicamenteuse, Bactérie Gram positif, Contrôle qualité, Europe, Assurance qualité, Etude multicentrique, Glycopeptide, Polypeptide, Evaluation, Méthodologie
Mots-clés Pascal anglais : Vancomycin, Teicoplanin, Antibiotic, Antibacterial agent, Sensitivity resistance, Drug susceptibility test, Gram positive bacteria, Quality control, Europe, Quality assurance, Multicenter study, Glycopeptide, Polypeptide, Evaluation, Methodology
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 98-0140552
Code Inist : 002B02S02. Création : 21/07/1998.