Study objective-To describe the epidemiology of Epstein-Barr virus (EBV) among primary school children by testing saliva with a new EBV capsid antigen « G » antibody capture radioimmunoassay (GACRIA).
Design-A population based sample of 7 year old schoolchildren were followed up at age 11.
Setting-30 randomly chosen primary schools in Edinburgh, Scotland.
Measurements-Data on risk factors for infection were collected by questionnaire at ages 7 and 11.
Saliva samples collected at age 11 were examined by GACRIA for evidence of previous infection with EBV.
For 102 subjects, a second salivary specimen collected approximately one month after the first sample was available for testing as a measure of the repeatability of the method.
Main results-Unequivocal results were found in 91% of samples and the repeatability of the test was good (K=0.71).
Fifty six per cent of children had antibodies to EBV.
In a logistic regression analysis, independent risk factors for infection were sharing a room (odds ratio 1.78,1.14,2.79), head of household's social class IV/V compared with I (odds ratio 2.87,1.08,7.34), and schools serving predominantly rented housing estates (odds ratio 2.3,1.09,4.84).
Conclusion-This study is the first application of EBV viral capsid GACRIA to salivary samples.
The method was successfully used to describe the epidemiology of EBV. (...)
Mots-clés Pascal : Virus Epstein Barr, Gammaherpesvirinae, Herpesviridae, Virus, Virose, Infection, Salive, Technique mesure, Diagnostic, Méthode radioimmunologique, Anticorps, Antigène G, Epidémiologie, Prévalence, Enfant, Homme, Préadolescent, Angleterre, Grande Bretagne, Royaume Uni, Europe
Mots-clés Pascal anglais : Epstein Barr virus, Gammaherpesvirinae, Herpesviridae, Virus, Viral disease, Infection, Saliva, Measurement technique, Diagnosis, Radioimmunoassay, Antibody, G antigen, Epidemiology, Prevalence, Child, Human, Preadolescent, England, Great Britain, United Kingdom, Europe
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 98-0136366
Code Inist : 002B05A02. Création : 21/07/1998.